A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.
Identifieur interne : 004A05 ( Main/Exploration ); précédent : 004A04; suivant : 004A06A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.
Auteurs : D S Hwang [États-Unis] ; A. KornbergSource :
- Cell [ 0092-8674 ] ; 1990.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Cartographie de restriction, Chromatographie d'affinité, Chromatographie d'échange d'ions, Chromosomes de bactérie, Cinétique, Deoxyribonuclease I, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Masse moléculaire, Plasmides, Protéines de liaison à l'ADN (isolement et purification), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN, Sondes oligonucléotidiques, Séquence nucléotidique.
- MESH :
- génétique : ADN bactérien, Escherichia coli.
- isolement et purification : Protéines de liaison à l'ADN.
- métabolisme : Escherichia coli, Protéines de liaison à l'ADN.
- Cartographie de restriction, Chromatographie d'affinité, Chromatographie d'échange d'ions, Chromosomes de bactérie, Cinétique, Deoxyribonuclease I, Données de séquences moléculaires, Masse moléculaire, Plasmides, Réplication de l'ADN, Sondes oligonucléotidiques, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Chromatography, Affinity, Chromatography, Ion Exchange, Chromosomes, Bacterial, DNA Replication, DNA, Bacterial (genetics), DNA-Binding Proteins (isolation & purification), DNA-Binding Proteins (metabolism), Deoxyribonuclease I, Escherichia coli (genetics), Escherichia coli (metabolism), Kinetics, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Restriction Mapping.
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , isolation & purification : DNA-Binding Proteins.
- chemical , metabolism : DNA-Binding Proteins.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- Base Sequence, Chromatography, Affinity, Chromatography, Ion Exchange, Chromosomes, Bacterial, DNA Replication, Deoxyribonuclease I, Kinetics, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Restriction Mapping.
Abstract
A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation.
DOI: 10.1016/0092-8674(90)90165-b
PubMed: 2208289
Affiliations:
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Le document en format XML
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Kornberg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2208289</idno><idno type="pmid">2208289</idno><idno type="doi">10.1016/0092-8674(90)90165-b</idno><idno type="wicri:Area/PubMed/Corpus">002A19</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A19</idno><idno type="wicri:Area/PubMed/Curation">002A19</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A19</idno><idno type="wicri:Area/PubMed/Checkpoint">002874</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002874</idno><idno type="wicri:Area/Ncbi/Merge">000900</idno><idno type="wicri:Area/Ncbi/Curation">000900</idno><idno type="wicri:Area/Ncbi/Checkpoint">000900</idno><idno type="wicri:doubleKey">0092-8674:1990:Hwang D:a:novel:protein</idno><idno type="wicri:Area/Main/Merge">004A83</idno><idno type="wicri:Area/Main/Curation">004A05</idno><idno type="wicri:Area/Main/Exploration">004A05</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.</title><author><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemistry, Stanford University School of Medicine</wicri:cityArea></affiliation></author><author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></author></analytic><series><title level="j">Cell</title><idno type="ISSN">0092-8674</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Chromatography, Affinity</term><term>Chromatography, Ion Exchange</term><term>Chromosomes, Bacterial</term><term>DNA Replication</term><term>DNA, Bacterial (genetics)</term><term>DNA-Binding Proteins (isolation & purification)</term><term>DNA-Binding Proteins (metabolism)</term><term>Deoxyribonuclease I</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Oligonucleotide Probes</term><term>Plasmids</term><term>Restriction Mapping</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Cartographie de restriction</term><term>Chromatographie d'affinité</term><term>Chromatographie d'échange d'ions</term><term>Chromosomes de bactérie</term><term>Cinétique</term><term>Deoxyribonuclease I</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Masse moléculaire</term><term>Plasmides</term><term>Protéines de liaison à l'ADN (isolement et purification)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Réplication de l'ADN</term><term>Sondes oligonucléotidiques</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Chromatography, Affinity</term><term>Chromatography, Ion Exchange</term><term>Chromosomes, Bacterial</term><term>DNA Replication</term><term>Deoxyribonuclease I</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Oligonucleotide Probes</term><term>Plasmids</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Chromatographie d'affinité</term><term>Chromatographie d'échange d'ions</term><term>Chromosomes de bactérie</term><term>Cinétique</term><term>Deoxyribonuclease I</term><term>Données de séquences moléculaires</term><term>Masse moléculaire</term><term>Plasmides</term><term>Réplication de l'ADN</term><term>Sondes oligonucléotidiques</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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